Gated STED -- developing the next generation of super-resolution microscopes

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Contact: Markus Berninger
Markus.Berninger@Max-Planck-Innovation.de
49-892-909-1930
Max-Planck-Gesellschaft

Leica Microsystems, the Max Planck Society and the German Cancer Research Center sign license agreement

This release is available in German.

Leica Microsystems has signed an agreement with the Max Planck Society and the German Cancer Research Center (DKFZ) for the development of the next generation of super-resolution STED (Stimulated Emission Depletion) microscopy. This gives Leica Microsystems the license to develop the new technology, called gated STED, into a commercial product and put it on the market.

Stefan Hell, Director at the Max Planck Institute for Biophysical Chemistry, has taken his idea of STED microscopy a momentous step further with gated STED: The new technology significantly improves the resolution and contrast previously attained with CW-STED (Continuous-Wave Stimulated Emission Depletion) microscopy, while distinctly reducing laser intensity. This enhances photostability as well as live cell capability, substantially extending the range of possible applications. Also, gated STED technology will considerably increase the number of issues that can be addressed with STED fluorescence correlation spectroscopy (STED-FCS). The main application of gated STED technology will be the observation of molecule movements in the membrane of living cells.

The new product of Leica Microsystems will be launched in the first half of the year 2012. Thanks to Leica Microsystems' modular concept, the Leica TCS SP5 and Leica TCS STED CW confocal systems already on the market can be upgraded with gated STED.

"We're delighted to be able to continue the provenly successful cooperation with the Max Planck Society, its technology transfer organization Max Planck Innovation and the DKFZ with this trailblazing product development," says Stefan Traeger, Vice President of Leica Microsystems' Life Science Division. "The new gated STED microscope will enable us to further strengthen our technological lead in super-resolution microscopy especially for confocal systems."

###



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AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.


[ Back to EurekAlert! ] Public release date: 11-Nov-2011
[ | E-mail | Share Share ]

Contact: Markus Berninger
Markus.Berninger@Max-Planck-Innovation.de
49-892-909-1930
Max-Planck-Gesellschaft

Leica Microsystems, the Max Planck Society and the German Cancer Research Center sign license agreement

This release is available in German.

Leica Microsystems has signed an agreement with the Max Planck Society and the German Cancer Research Center (DKFZ) for the development of the next generation of super-resolution STED (Stimulated Emission Depletion) microscopy. This gives Leica Microsystems the license to develop the new technology, called gated STED, into a commercial product and put it on the market.

Stefan Hell, Director at the Max Planck Institute for Biophysical Chemistry, has taken his idea of STED microscopy a momentous step further with gated STED: The new technology significantly improves the resolution and contrast previously attained with CW-STED (Continuous-Wave Stimulated Emission Depletion) microscopy, while distinctly reducing laser intensity. This enhances photostability as well as live cell capability, substantially extending the range of possible applications. Also, gated STED technology will considerably increase the number of issues that can be addressed with STED fluorescence correlation spectroscopy (STED-FCS). The main application of gated STED technology will be the observation of molecule movements in the membrane of living cells.

The new product of Leica Microsystems will be launched in the first half of the year 2012. Thanks to Leica Microsystems' modular concept, the Leica TCS SP5 and Leica TCS STED CW confocal systems already on the market can be upgraded with gated STED.

"We're delighted to be able to continue the provenly successful cooperation with the Max Planck Society, its technology transfer organization Max Planck Innovation and the DKFZ with this trailblazing product development," says Stefan Traeger, Vice President of Leica Microsystems' Life Science Division. "The new gated STED microscope will enable us to further strengthen our technological lead in super-resolution microscopy especially for confocal systems."

###



[ Back to EurekAlert! ] [ | E-mail | Share Share ]

?


AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.


Source: http://www.eurekalert.org/pub_releases/2011-11/m-gs111111.php

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Lion tranquilized outside den at Seattle zoo

(AP) ? A lion got out of its den at the Woodland Park Zoo in Seattle, but it didn't get far before it was tranquilized in a service building.

The zoo says the 12-year-old female named Kalisa gained access to a hallway Tuesday afternoon. The zoo's emergency response team of firearms units and veterinary staff immediately responded with tranquilizer darts.

She was secured in about an hour. Two other lions remained in their dens during Kalisa's brief escape.

The zoo is investigating how she made her break.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/aa9398e6757a46fa93ed5dea7bd3729e/Article_2011-11-09-Lion%20Recaptured/id-45b036c72aac4b5caede5f4dd588ac05

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Obama seeks Russia, China help on Iran (AP)

HONOLULU ? Searching for help, President Barack Obama lobbied the skeptical leaders of Russia and China on Saturday for support in keeping Iran from becoming a nuclear-armed menace to the world, hoping to yield a "common response" to a deepening problem.

Yet Obama's talk of solidarity with Russian President Dmitry Medvedev and Chinese President Hu Jintao was not publicly echoed by either man as Iran moved anew to the fore of the international stage ? and to the front of the fierce U.S. presidential race.

The United States' vast worries about Iran grew starker with a report this week by the U.N. atomic agency that asserted in the strongest terms yet Iran is conducting secret work with the sole intent of developing nuclear arms. The U.S. claims a nuclear-armed Iran could set off an arms race among rival states and directly threaten Israel.

Russia and China remain a roadblock to the United States in its push to tighten international sanctions on Iran. Both are veto-wielding members of the U.N. Security Council and have shown no sign the new report will change their stand.

With Medvedev on the sidelines of an Asia-Pacific summit here, Obama said the two "reaffirmed our intention to work to shape a common response" on Iran.

Shortly after, Obama joined Hu, in a run of back-to-back diplomacy with the heads of two allies that hold complicated and at times divisive relations with the United States.

Speaking to reporters before he talked to Hu, Obama said that he and the Chinese leader want to ensure that Iran abides by "international rules and norms."

Obama's comments were broad enough to portray a united front without yielding any clear indication of progress. Medvedev, for his part, was largely silent on Iran during his remarks, merely acknowledging that the subject was discussed. Hu did not mention Iran at all.

As the president held forth on the world stage in his home state, Republicans vying to compete against Obama for the presidency unleashed withering criticism in a debate in South Carolina. It was a rare moment in which foreign policy garnered attention in a campaign dominated by the flagging U.S. economy.

"If we re-elect Barack Obama, Iran will have a nuclear weapon. And if you elect Mitt Romney, Iran will not have a nuclear weapon," said Romney, the former Massachusetts governor. Minnesota Rep. Michele Bachmann warned that Iran's attempt to develop a nuclear weapon is setting the table "for worldwide nuclear war against Israel."

Iran has insisted its nuclear work is in the peaceful pursuit of energy and research, not weaponry.

U.S. officials have said the report by the International Atomic Energy Agency was unlikely to persuade China and Russia to support tougher sanctions on the Iranian government. But led by Obama, the administration is still trying to mount pressure on Iran, both through the United Nations and its own, for fear of what may come should Iran proceed undeterred.

More broadly, Obama sought Saturday to position the United States as a Pacific power determined to get more American jobs by tapping the explosive potential of the Asia-Pacific.

For businesses, he said, "this is where the action's going to be."

"There is no region in the world that we consider more vital than the Asia-Pacific region," he told chief executives gathered for a regional economic summit.

The president went so far as to saying the United States had grown "a little bit lazy" in trying to attract business to the United States.

Underscoring the search for some good economic news ahead heading toward a re-election vote, Obama announced the broad outlines of an agreement to create a transpacific trade zone encompassing the United States and eight other nations. He said details must still be worked out, but said the goal was to complete the deal by next year.

"The United States is a Pacific power and we're here to stay," Obama said.

The eight countries joining the U.S. in the zone would be Australia, Brunei, Chile, Malaysia, New Zealand, Peru, Singapore and Vietnam. A central topic for Obama and Japanese Prime Minister Yoshihiko Noda was Japan's interest in joining the trade bloc.

In a sign of potential tension with China, Mike Froman, a deputy national security adviser who focuses on international economic matters, shrugged off complaints from China that it had not been invited to join the trade bloc.

He told reporters that China had not expressed interest in joining and said the trade group "is not something that one gets invited to. It's something that one aspires to."

Addressing the European debt crisis, Obama said he welcomed the new governments being formed in Greece and Italy, saying they should help calm world financial markets.

Medvedev thanked Obama for his support in Russia's expected entrance into the World Trade Organization, asserting that Russia has received more help from this administration than all previous ones. Russia is expected to join the WTO next year, a step that would require Congress to approve permanent normal trade relations.

Obama is the host of the APEC gathering, a non-binding forum that draws 21 nations from across a vast Asia-Pacific region.

Obama will be in Honolulu through Tuesday, when he leaves for Australia before ending his trip in Indonesia, the country where he spent several years as a boy, for a security summit of Asian nations.

___

Associated Press Writer Erica Werner contributed to this report.

Source: http://us.rd.yahoo.com/dailynews/rss/topstories/*http%3A//news.yahoo.com/s/ap/20111113/ap_on_go_pr_wh/us_obama

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Incumbent parties hang on in Kentucky, Mississippi

Firefighters, police officers and supporters cheer Tuesday night, Nov. 8, 2011, in Youngstown, Ohio, after Ohio voters defeated the state's new collective bargaining law. The law would have limited the bargaining abilities of 350,000 unionized public workers. (AP Photo/The Vindicator, Robert K. Yosay)

Firefighters, police officers and supporters cheer Tuesday night, Nov. 8, 2011, in Youngstown, Ohio, after Ohio voters defeated the state's new collective bargaining law. The law would have limited the bargaining abilities of 350,000 unionized public workers. (AP Photo/The Vindicator, Robert K. Yosay)

Ohio Gov. John Kasich speaks about Issue 2 and election results at a news conference Tuesday, Nov. 8, 2011, in Columbus, Ohio. In a political blow to Kasich, voters handily rejected the state's new law, which would have limited the bargaining abilities of 350,000 unionized public workers. (AP Photo/Jay LaPrete)

Supporters against Senate Bill 5 celebrate after the bill was defeated Tuesday, Nov. 8, 2011, in Columbus, Ohio. Ohio voters on Tuesday rejected a new law restricting the collective bargaining abilities of public employee unions in an unusually vigorous off-year election that drew attention across the nation. Voters also approved a constitutional amendment intended to keep government from requiring Ohioans to participate in any health care system. (AP Photo/Tony Dejak)

KentucyGov. Steve Beshear, second from right, and his running mate Jerry Abramson celebrate their victory with their wives Madeline Abramson, left, and Jane Beshear, right, in Frankfort, Ky., Tuesday Nov. 8, 2011. Beshear was re-elected Tuesday, becoming the second Democrat to win a U.S. gubernatorial race this year amid lingering economic uncertainty that's already proving worrisome to President Barack Obama's 2012 effort. (AP Photo/John Flavell)

Governor-elect Phil Bryant shares a laugh with Judy Mixon of Hattiesburg, as he and his wife Deborah Bryant, center leave a victory party Tuesday, Nov. 8, 2011 in Jackson, Miss. (AP Photo/Rogelio V. Solis)

For all the frustration surrounding the economy, voters refused to throw incumbent parties out of governors' and most big-city mayors' offices, and they turned back an Ohio law that aimed to ease grinding budget problems by restricting the union rights of public employees.

In the heart of the Bible Belt, a Mississippi initiative that would have defined life as beginning at fertilization also went down to defeat, ending a plan to use it to challenge Roe v. Wade, the 1973 Supreme Court decision that established the right to abortion.

Across the nation, voters' last major judgments of 2011 were sure to be closely analyzed for any clues about the public's political mood just two months ahead of the first presidential primary and nearly four years into the worst economic slowdown since the Depression.

Kentucky's Democratic governor easily won another term, and Mississippi voters kept their governor's office in GOP hands ? decisions that suggested many Americans were not ready to abandon the parties in power.

In Ohio, a hotly debated new law that severely limited the bargaining rights of more than 350,000 teachers, firefighters, police officers and other public employees was repealed with more than 60 percent of the vote. The defeat was a stinging blow to Gov. John Kasich and cast doubt on other Republican governors who have sought union-limiting measures as a means to curb spending.

"Ohio sent a message to every politician out there: Go in and make war on your employees rather than make jobs with your employees, and you do so at your own peril," AFL-CIO President Richard Trumka said.

Kasich congratulated his opponents and pledged to consider his next steps carefully.

"I've heard their voices. I understand their decision, and frankly, I respect what people have to say in an effort like this," he said, adding that the vote requires him "to take a deep breath" and "spend some time reflecting on what happened here."

The disputed law permitted workers to negotiate wages but not pensions or health care benefits, and it banned public-worker strikes, scrapped binding arbitration and eliminated annual raises for teachers.

The outcome will no doubt be studied by presidential candidates as a gauge of the Ohio electorate, which is seen as a bellwether. No Republican has won the White House without Ohio, and only two Democrats have done so in more than a century.

Elsewhere on the ballot, Ohio voters approved a proposal to prohibit people from being required to buy health insurance as part of the national health care overhaul. The vote was mostly symbolic, but Republicans hoped to use it in a legal challenge.

The governors' races were of keen interest to both parties. Ten states will elect governors next year, and governors can marshal get-out-the-vote efforts crucial to any White House candidate. The first presidential primary is Jan. 10 in New Hampshire.

In Kentucky, Gov. Steve Beshear was easily re-elected despite high unemployment, budget shortfalls and an onslaught of third-party attack ads. He became the second Democrat to win a governor's race this year, after West Virginia's Earl Ray Tomblin.

In Mississippi, voters picked Republican Lt. Gov. Phil Bryant to succeed Haley Barbour, who could not run again because of term limits. Bryant beat Hattiesburg Mayor Johnny Dupree, the first black major-party nominee for governor in Mississippi.

The Mississippi measure to define life as beginning at fertilization would have been the first victory in the country for the so-called personhood movement, which aims to make abortion all but illegal. Similar attempts have failed in Colorado and are under way elsewhere.

The proposal divided the medical and religious communities and caused some of the most ardent abortion opponents, including Barbour, to waver in their support.

Opponents said the measure would have made some forms of birth control, such as the morning-after pill or the intrauterine device, illegal. And they worried that it could have deterred physicians from performing in vitro fertilization for fear of criminal charges if an embryo did not survive.

In Arizona, state Sen. Russell Pearce, architect of the tough immigration law that put the state at the forefront of the national debate, was ousted after a recall attempt led by a fellow Republican.

Other votes of note:

? Hundreds of cities held mayoral races, including some of the nation's largest. In San Francisco, interim Mayor Ed Lee had a strong lead in early returns and would become the city's first elected Asian-American mayor if he wins. But it could be days before final results are known because of a complicated system in which voters rank their top three candidates.

In Philadelphia, Democratic Mayor Michael Nutter trounced a little-known Republican challenger.

Phoenix elected Democrat Greg Stanton, a former city council member, as its new mayor after a campaign focused on pulling the nation's sixth-largest city out its economic and foreclosure slump.

Incumbent mayors also prevailed in Baltimore and Indianapolis.

? Comic-turned-politician Robert Farmer lost his bid to become Kentucky's agriculture commissioner. Farmer told hillbilly jokes that upset some people, and he had no farming experience. In Ohio, another comedian, Drew Hastings, a fixture on "Comedy Central," became mayor of tiny Hillsboro.

? In Maine, voters repealed a new state law that required voters to register at least two days before an election. The decision restored Election Day voter registration, which had been available for nearly four decades. A proposal to allow casinos in certain communities was rejected.

? Washington state voters approved a plan to end the state-run liquor system and allow large stores to sell alcohol. The proposal was bankrolled by giant retailer Costco, which spent more than $22 million, making it the costliest initiative in Washington history.

? Atlanta overwhelmingly approved Sunday alcohol sales, clearing the way for shoppers to buy liquor in stores as soon as New Year's Day.

? Oregon held a special primary to replace Democratic Rep. David Wu, who resigned in August after being accused of an unwanted sexual encounter with an 18-year-old woman. Wu was the fourth member of Congress to quit this year in a sex scandal.

___

Associated Press Writer Julie Carr Smyth in Columbus, Ohio, contributed to this report.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/89ae8247abe8493fae24405546e9a1aa/Article_2011-11-09-Election%20Rdp/id-f72d05d21e2d492ca251fa63d0292eeb

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New Madonna song leak has singer 'upset'

Madonna's manager Guy Oseary has said the singer was "very upset" that a demo of her new song "Give Me All Your Love" had leaked onto the Internet.

The song, which hit the web on Wednesday and has been listened to by fans around the world, is expected to be the debut track from Madonna's upcoming album, her first studio release since leaving Warner Bros to join Live Nation.

"Madonna told me this morning 'my true fans wouldn't do this'... whoever is responsible for this leak, we ask that you please stop!" Oseary wrote via Twitter.

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"I'm very happy with the positive reaction to the demo, but we are very upset with whoever leaked the song!!!!!!!!" he added.

Addressing questions tweeted by fans, Oseary said the new album should be finished "in the next month or so," and that it did not yet have a title.

Slideshow: Madonna (on this page)

He added that the 53 year-old star, whose hits include "Like a Virgin," "Vogue" and "Hung Up," had penned a "beautiful ballad" for her movie "W.E.."

The picture, Madonna's second feature film as director, follows the life of Wallis Simpson, the American divorcee whose relationship with King Edward VIII sparked a constitutional crisis in Britain and led to his abdication.

Story: 10 Unforgettable MTV Moments

The film, which had its world premiere at the Venice film festival, hits U.S. theatres in December and British cinemas in January.

Madonna's new record will be her first studio album since 2008's "Hard Candy," which debuted at No. 1 on the Billboard 200 chart in the United States.

Story: Marilyn Monroe film dress fetches $504,000

In 2007, Madonna dropped her long-time music label Warner Bros and signed a deal with concert promoter Live Nation reported to be for 10 years and worth $120 million.

The deal, part of a recent trend in the struggling music industry, involved not only record sales, but also touring and merchandising.

Source: http://today.msnbc.msn.com/id/45238543/ns/today-entertainment/

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Blogger on cartel beheading: 'Cannot kill us all'

Another blogger has been decapitated, purportedly in retaliation for postings about drug cartels, prompting users of social network sites to unite in their stance against the gangs.

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The gruesome slaying on Wednesday is believed to be the fourth since early September in which a drug cartel killed people in Nuevo Laredo for what they said online.

"This happened to me for not understanding that I shouldn't report on the social networks," said a placard left with the man's body at a busy intersection in Nuevo Laredo, according to The Houston Chronicle.

Nuevo Laredo, across the border from Laredo, Texas, has been dominated for about the last two years by the violent Zetas drug cartel.

The victim was described as 35 and identified on social networking sites by the nickname El Rascatripas or "belly scratcher."

The victim reportedly posted updates on the Zetas' activities and had collaborated with slain journalist Mary Elizabeth Macias, 39, who was butchered in the same manner and dumped in the same spot, El Universal.com reported.

Bloggers and users of online chatrooms reacted with shock.

"I'm doing some digging on it now," blogger Oscar Villanueva emailed msnbc.com on Wednesday. "I am also helping and working with my Twitter circle in creating a Twitter manifesto, calling out for us to unite, continue denouncing, and we will be offering tips on how to continue doing so safely and effectively."

Villanueva, who blogs on Borderland Beat, added, "THESE DEATHS WILL NOT BE IN VAIN...They cannot kill us all!!"

Mexican citizens have been increasingly relying on social media chatrooms and sites, such as Facebook and Twitter, as traditional media self-censor in the face of cartel violence.

Bloggers who cover cartels have been increasingly at risk.

In September, police found Macias' decapitated body alongside a handwritten sign saying she was killed in retaliation for postings on a social networking site. The message was signed with a "Z," the Zetas' trademark.

Earlier that month, the bodies of a man and a woman were found dangling from an overpass in Nuevo Laredo with a message threatening, "this is what will happen" to trouble-making Internet users.

'Killings, torture'
But according to a new report, the cartels are not the only side committing atrocities in Mexico's drug war, which President Felipe Calderon launched in late 2006.

Human Rights Watch in an investigation released Wednesday accused the Mexican government of torture, forced disappearances and extra-judicial killings in its war against organized crime.

The report outlines misconduct at all levels of authority, from prosecutors who give detainees prewritten confessions to sign, to medical examiners who classify beatings and electric shock as causing minor injuries.

Only 15 soldiers have been convicted out of the 3,671 investigations launched by military prosecutors into alleged human rights violations by soldiers against civilians from 2007 to June 2011, according to the report. Not a single soldier or state official has been convicted in any of more than 200 cases the New York-based organization documented in the report.

The report says it documented 170 cases with credible evidence of torture, including waterboarding, electric shocks and asphyxiation, 39 forced disappearances and 24 cases of extra-judicial killings by security forces. The investigators said they only used cases in which victims' accounts could be corroborated by eyewitnesses, medical reports, coinciding testimony by people with no connections to each other or official investigations.

Human Rights Watch investigators met with Calderon, the country's interior secretary, attorney general and leaders of the armed forces to present the report. Calderon said in a statement Wednesday that he would form a joint working group with Human Rights Watch to analyze the findings.

But he added that criminals are the biggest threat to the human rights of Mexicans and said his government has the legal and ethical obligation to employ every method at its disposal to establish authority in communities where drug gangs are warring.

The drug war had claimed more than 35,000 lives by the end of 2010. The government hasn't issued new figures since then, although news media and other groups put the number at more than 43,000.

Source: http://www.msnbc.msn.com/id/45230870/ns/world_news-americas/

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FCC Fridays: November 11, 2011

We here at Engadget Mobile tend to spend a lot of way too much time poring over the latest FCC filings, be it on the net or directly on the ol' Federal Communications Commission's site. Since we couldn't possibly (want to) cover all the stuff that goes down there, we've gathered up all the raw info you may want (but probably don't need). Enjoy!

Phones

Fujitsu FJI12
LG C800G
Research in Motion REC70UW
Research in Motion RED70UW
Research in Motion RDZ20CW
Samsung Galaxy S II LTE SC-03D (NTT DoCoMo, Dark Gray)
Samsung Galaxy S II LTE SC-04D (NTT DoCoMo, Titan Silver)
Samsung Galaxy W (GT-I8150T)
Samsung Galaxy XCover (GT-S5690)
Samsung GT-S5369
Sharp 102SH
Sharp SH-02D (NTT DoCoMo)
Sharp SH-03D (NTT DoCoMo)
ZTE GR221S
ZTE N762

Tablets and peripherals

Acer Iconia Tab A200
Barnes & Noble Nook Color
Huawei E303s-3 USB stick
Motorola LapDock 500
Samsung Galaxy Tab 7.0 Plus (T-Mobile)
ZTE Light (V9C)

FCC Fridays: November 11, 2011 originally appeared on Engadget on Fri, 11 Nov 2011 08:55:00 EDT. Please see our terms for use of feeds.

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Source: http://feeds.engadget.com/~r/weblogsinc/engadget/~3/9jJ7DWfA_g8/

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93% Take Shelter

schizophrenia or prophecy? this is a hard film to score. its at least a 4 star film, and potentially 5, so ill go in between. it doesnt have a high re-watchability factor which is my only criticism, and that isnt really a criticism because its more a product of the material then the quality of the film. this is easily one of the best films of the year, and one of the better films in the past few years. shannon and chastain were both amazing in their roles, which for chastain amazes me because she has so little experience in film, and even the supporting players around them all added wonderful performances. the subject matter is as engaging as it gets, and as i hoped for through the entire film, things are not what they seem. beautiful direction by nichols, amazing cinematography for such a small scale story, and the material was handled with perfect care. nichols, shannon, and chastain probably all deserve oscar noms, and this is the type of soul stirring film that must be seen. i havent felt like this leaving a film since "a serious man".

November 7, 2011

Source: http://www.rottentomatoes.com/m/take_shelter/

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